Cavtratin reverses effects of CCL2 and Ad-siCav-1 on TJ- and AJ-associated proteins. Western blotting (A) and corresponding quantification (B) for Ad-siCav-1 effects. BMECs were transduced with Ad-siCav-1 or Ad-siLuc (control) as in Figure 5. After 48 hours of viral exposure, 2 μM cavtratin was added to both the top and bottom Transwell chambers and cells wells cultured for an additional 48 hours. *P < .01 when compared with Ad-siCav-1–treated cells. Western blotting (C) and corresponding quantification (D) for CCL2 effects. BMECs were cultured to confluence, pretreated with 2 μM cavtratin for 2 hours, and then exposed to 10 nM CCL2 for 18 hours (both cavtratin and CCL2 were added to top and bottom Transwell chambers). *P < .01 when compared with cells treated with CCL2 only.