Ad-siCav-1 promotes CCL2-stimulated monocytic migration across BMECs. BMECs were transduced with Ad-siCav-1 or Ad-siLuc (control) and, after 96 hours of viral exposure, WEHI cells were added to the top Transwell chamber and CCL2 was (10 nM) applied to the lower chamber of all samples. At the indicated time points, transmigrated cells were collected from the lower chamber and quantified as detailed in “Materials and methods.” Relative transmigration was calculated as x-fold increase above 3 hours of control. The data are presented as mean ± SE and represent 3 independent experiments performed in triplicate. *P < .01 when compared with its respective control.