Ad-siCav-1 knocks down caveolin-1 expression in BMEC monolayers. Just subconfluent BMECs were transduced with Ad-siCav-1 as described in “Materials and methods.” (A) Western blotting showing dosage (top; 96 hours) and time course (bottom; 500 ifu per cell) effects of Ad-siCav-1 exposure. Ad-siLuc refers to an adenoviral construct expressing luciferase siRNA, which served as a control for Ad-siCav-1. An inoculum of 500 ifu per cell produced the most dramatic effect, which was achieved after 96 hours of viral exposure. (B) Immunofluorescent staining with antibody to caveolin-1 of BMEC monolayers transduced with Ad-siCav-1 (500 ifu per cell, 96 hours). A significant reduction in staining intensity is readily apparent. Scale bar represents 50 μm.