Biodistribution of AAV vector following mesenteric and peripheral vein administration of scAAV2/8-LP1-hFIXco. (A) Results of semiquantitative PCR analysis in which 1 μg genomic DNA, isolated from the indicated organs at 4 weeks after administration of 1 × 10¹² vg/kg scAAV2/8 particles via the mesenteric (top 2 panels) or peripheral venous route, was subjected to PCR using primers unique to hFIXco and designed to amplify a 617 bp product. Integrity of the DNA was determined by amplifying a 604 bp region of the rhesus β-actin gene and is shown at the bottom of each panel. (B) Q-PCR reactions were performed in duplicate to quantitate transgene copy number in each organ after peripheral (⊡, n = 3) and mesenteric (□, n = 2) vein administration of scAAV2/8. The results are represented as vector copy number per diploid genome together with standard errors of mean.