Ets-1 induces PDGF-D expression from mRNA level and transcriptional level. (A) SMCs were transfected with 30 μg Ets-1 expression vector and backbone vector pcDNA3. After 24 hours, total RNA was extracted and semiquantitative RT-PCR (left) or real-time PCR (right) was performed. (B) SMCs were cotransfected with 50, 125, and 500 ng of the Ets-1 expression vector or the corresponding amount of pcDNA3 together with 10 μg p1168PDGF-Dluc plasmid. (C) SMCs were cotransfected with 3 μg Ets-1 plasmid or dominant-negative Ets-1 (DN-Ets-1), respectively, together with 10 μg p1168PDGF-Dluc. Luciferase activity was determined after 24 hours. The data are representative of at least 2 independent experiments. Error bars represent SEM performed in duplicate or triplicate. *P < .05 relative to control using Student t test.