Surface immunophenotype of LECs and KLECs. (A) Surface expression levels of proteins involved in antigen presentation in LECs (⊡) and GFP-expressing KLECs (▪) were assessed by flow cytometry 4 days after infection. Error bars correspond to standard deviation, calculated after 3 independent infections with different batches of KSHV. Significant changes are indicated with asterisks (P values were calculated with a 2-sided t test). A representation of the corresponding GEM data are also shown (significantly up- and down-regulated gene names are shown in red and blue, respectively, whereas black corresponds to transcripts that were not significantly affected after infection). (B) HLA-A mRNA levels in LECs and GFP-expressing KLECs (purified by cell sorting, 4 days after infection) were determined by qRT-PCR. mRNA levels are normalized to LEC. A heat map of GEM data for HLA-A (2 probe-sets), HLA-B (1 probe-set), and HLA-C (7 probe-sets) in 6 pairs of LECs and KLECs is also shown. The heat map color scale indicates units of standard deviation from the mean expression of each row (red high and blue low expression).