Contribution of p110γ and p110δ activity in thymocyte development in vitro. (A) Representative flow cytometry profiles of fetal thymic organ cultures harvested from day 14.5 WT, p110δ^-/-, and p110γ^-/- embryos that were treated with either vehicle control or p110δ-specific inhibitor IC87114 (10 μM) for 1 week. (B) Percentage reduction in DP thymocyte population after treatment with IC87114 compared with control treatment (mean ± SE; n = 3).