Role of PI3Kδ and PI3Kγ in thymocyte development. Flow cytometry analysis of expression of CD4 and CD8 on total thymocyte population (A), CD25 and CD44 on DN thymocytes (B), intracellular TCRB in CD25⁺ DN thymocytes (C), and TCRB on the surfaces of DP (D) and CD8⁺ (E) thymocytes. Percentage of gated cells in a particular quadrant is indicated. Data are representative of 3 independent experiments. Total thymocyte counts are in bold (mean ± SE; n = 5).