Response of the hTLR2-transfected TLR2^−/−EFs to APLA5 and β2GP1-IgG. The cells were stimulated for 8 hours with 500 μg/mL APLA from patient 5 or control IgG, or with 25 μg/mL β2GP1-IgG from patient 6. The results show mRNA levels of MCP-1, ICAM-1, and IL-6 quantified by qRT-PCR. Data are mean value ± SE of 5 (APLA5) to 9 (β2GP1-IgG) independent experiments.