Transgenic PAI-1 expression reduces distant organ injury duringKlebsiella pneumonia. Representative HE stainings of liver (A-B) tissue at 48 hours after intranasal inoculation of 10⁴ CFU K pneumoniae. Mice were pretreated with 5 × 10⁸ PFU Ad.PAI-1 or Ad.RR5 intranasally at 24 hours before infection. Magnification, × 200. Livers of Ad.RR5-injected mice (A) showed inflammation, necrosis (*), and thrombi (arrow). (C) Graphic representation of the degree of liver damage at 48 hours after infection, determined according to the scoring system described in “Histologic examination.” ASAT (D), ALAT (E), urea (F), and creatinine (G) were measured in plasma at the same time point. Data are means ± SE of 8 mice per genotype. *P < .05, **P < .01 versus Ad.RR5-treated mice.