Transgenic PAI-1 expression reduces lung inflammation during Klebsiella pneumonia. Representative HE stainings of lung (A-B) tissue at 48 hours after intranasal inoculation of 104 CFU K pneumoniae. Mice were pretreated with 5 × 108 PFU Ad.PAI-1 or Ad.RR5 intranasally at 24 hours before infection. Magnification, × 200. Insets show representative neutrophil stainings of the same time point; magnification, × 200. (C) Graphic representation of the degree of lung inflammation, determined according to the scoring system described in “Histologic examination.” (D) MPO levels were determined at 24 and 48 hours after infection. Data are means ± SE. n = 8 per group at each time point. *P < .01, **P < .001 versus Ad.RR5-treated mice at the same time point.
