Influence of PAI-1 deficiency on lung inflammation during Klebsiella pneumonia. Representative HE stainings of lung tissue of Wt (A) and PAI-1−/− (B) mice at 48 hours after intranasal inoculation of 104 CFU K pneumoniae. Original magnification, × 100. Insets show representative neutrophil stainings at the same time point; original magnification, × 200. (C) Graphic representation of the degree of lung inflammation, determined according to the scoring system described in “Histologic examination.” As a reference, normal mouse lung tissue is shown in panel D. (E) MPO levels and (F) plasma KC levels were determined at 24 and 48 hours after infection. (C,E-F) Data are means ± SE. n = 8 per group at each time point. *P < .05 versus Wt mice at the same time point.
