Double immunofluorescence labeling of lymphoid tissue to show interfollicular B cells. Top panel: The low-power view (left) shows B cells (labeled in red for CD20) and CD8⁺T cells (green). Scattered B cells are present in the interfollicular area adjacent to a B cell follicle (Foll). The high-power view (right) shows dendritic processes extending from several of these interfollicular B cells (arrow) and a single example of an interfollicular B cell (asterisk) in intimate contact with a CD8 cell. Bottom panel: Double immunofluorescence labeling for CD20 (green) and the dendritic cell marker CD208/DC-LAMP (red) shows that these 2 cell populations are clearly distinct. In the high-power view (right), typical interfollicular dendritic B cells are seen (eg, in the inset and as indicated by an arrow). Adapted from Marfioti et al¹ (used with permission).