EM011 causes morphologic changes in cells and increases TUNEL⁺ staining. Untreated CEM and CEM/VLB100 cells (A-B) show normal microtubule arrays and nuclei in interphase and display bipolar spindles in mitosis (insets). Prolonged EM011 treatment causes devastating morphologic changes and induces the formation of apoptotic bodies (C-D). (E-H) Quantitation of apoptosis by TUNEL analysis in untreated control cells (E-F) and EM011-treated cells (G-H). Scale bar = 40 μm. Results are representative of 2 independent experiments performed in triplicate.