FLT3 TKI-resistant M14(R)5214 and M14(R)701 cell lines maintain higher expression levels of 5 genes regulated by FLT3 signaling and required for FLT3-mediated cellular transformation despite FLT3 inhibition. Molm14, M14(R)5214, and M14(R)701 cell lines were treated with 50 nM CEP-701 for 4 hours prior to isolation of total RNA. The RNA was converted into cDNA and used for Q-PCR analysis. Target gene expression was normalized to GAPDH.