Spi-B rescues pDC development blocked by icNotch1 but not by GATA-3. (A) CD34⁺CD1a^- cells were double transduced with icNotch1 and Spi-B or control virus and cocultured with OP9-control cells (n = 4). (B) CD34⁺CD1a^- cells were double transduced with GATA-3 and Spi-B or control virus and cocultured with OP9-control cells (n = 3). Cultures were analyzed after 1 week for the presence of CD123^hiBDCA2⁺ pDC. Dot plots shown are gated on double transduced GFP⁺YFP⁺ cells. Experiments representative of at least 3 experiments are shown.