Functionally active anti-Aspergillus CD4⁺ T cells are enriched after selection and culture. (A) IFN-γ (top row) and IL-2 (bottom row) staining of anti-Aspergillus CD4⁺ lymphocytes from 1 donor. (B) Percentage of IFN-γ-positive anti-Aspergillus CD4⁺ lymphocytes from 7 of 7 different donors. Cells were stimulated with unloaded autologous APCs (first column) or autologous APCs prestimulated with either control antigen (tetanus toxoid; second column) or EC SAB (third column). The horizontal bars represent the mean.