Reverse effects of PI3K and GSK-3 on IL-12 and IL-10 secretion. Immature MoDCs were prepared and activated as in Figure 2. SB415286 (10 μM) and wortmannin (WM, 100 ng/mL) were added 20 minutes prior to activation; E coli were used in 2 concentrations (1:1 and 1:100; “Materials and methods”). Supernatants were harvested 36 hours after activation. Shown are means ± SEM; n = 8 (E coli, 1:1) and n = 4 experiments (E coli, 1:100). **P < .01 (comparison of WM vs WM + SB415286 only).