GSK-3 enhances proinflammatory cytokine secretion by CD40L-activated DCs and reduces migration of E coli–activated DCs. Immature MoDCs were prepared as in Figure 2–3. Cells were either continued in GM-CSF + IL-4 (no activation), or activated with a baby hamster kidney cell line transfected with CD40L (BHK-CD40L) at a 20:1 ratio of MoDCs to BHK-CD40L cells with or without LiCl (10 mM) or with or without SB415286 (10 μM). (A) Cytokine levels in MoDC culture supernatants 36 hours after activation. Shown are means ± SEM; n = 6. *P < .05, **P < .01 compared with activation with the BHK-CD40L cell line. (B) Migration toward CCL21 (40 nM) of MoDCs activated with BHK-CD40L cells and the cAMP-analog Sp-5,6-DCl-cBIMPS (cBIMPS, 50 μM) or with E coli as in Figure 3. Migration was tested in transwell assays with a pore size of 5 μm. Number of separate donors: BHK-CD40L + cBIMPS, n = 6; E coli, n = 15. **P < .01 compared with activation without inhibitor. (C) Expression of CCR7 36 to 38 hours after activation of MoDCs with or without SB415286. Mean fluorescence values relative to DCs activated with BHK-CD40L + cBIMPS (highest levels). Shown are means ± SD; n = 8. *P < .05, **P < .01 compared with immature DCs without the inhibitor.