Quantitative real-time RT-PCR analysis of gene expression repressed in response to retroviral-mediated gene transfer of THAP1 into primary human ECs. RNA samples from HUVECs transduced with pMLV-MCS (HUVEC-MCS) or pMLV-THAP1 (HUVEC-THAP1) retroviral expression vectors were analyzed by qPCR. The fold change in the mRNA levels for 15 selected genes (identified in the DNA microarray experiments; Table 1), between HUVEC-MCS and HUVEC-THAP1, was calculated. The mean and standard error for 2 independent data sets are shown (Q, ▪). For comparison, the fold changes obtained in the DNA microarray experiments (P < .01) are indicated (M, □). The levels of down-regulation observed in THAP1-expressing ECs were generally higher in the qPCR experiments than in the microarray experiments.