Lin⁻CD34⁺CD38⁻Rho^lo cells provide a significantly higher level of human engraftment than Lin⁻CD34⁺CD38⁻Rho^hi cells in NOD/SCID mice. (A) Lineage and CD38-depleted CB cells were sorted into Lin⁻CD34⁺CD38⁻Rho^lo and Lin⁻CD34⁺CD38⁻Rho^hi fractions. The gates for Rho sorting were set as the bottom and top 20% of Rho uptake within the Lin⁻CD34⁺CD38⁻ population. Equal numbers of cells (3500 per mouse) from sorted fractions were transplanted and mice were evaluated for erythroid (CD45⁻CD36⁺GlyA⁺, ▨) and lymphomyeloid (CD45⁺, ▪) human engraftment at 3 weeks (n = 21 mice) (B) and lymphomyeloid engraftment at 7 weeks (n = 16 mice) (C) in both the injected right femur (RF) and remaining bone marrow (BM). (*) P < .05 versus Lin⁻CD34⁺CD38⁻Rho^hi cells for CD45⁺ grafts in both panels B and C. Error bars represent SE.