Characterization of BM-derived WT and BLT1/2^−/− DCs. (A) FACS analysis of membrane phenotype in WT and BLT1/2^−/− DCs. Gray line indicates isotype control; broken line, iDCs; and black line, mDCs. (B) FITC-dextran uptake. (C) Mixed leukocyte reaction induced by WT and BLT1/2^−/− DCs. (D,E) Calcium mobilization measurement in response to LTB₄ in WT (D) and in BLT1/2^−/− (E) DCs. (F, G) In vitro chemotaxis of iDCs (F) and mDCs (G) toward CCL3 (100 ng/mL), CCL19 (100 ng/mL), and LTB₄ (100 nM). Error bars indicate SE.