Retroviral introduction of both an exogenous TCR-α and TCR-β chain in RCD cell lines P2 and P3 restores TCR-αβ surface expression. FACS analysis after retroviral transduction of cells from RCD cell line P3 with the TCR-α and/or TCR-β chain from T-cell clone N10. (Top panel) GFP- (TCR-α from N10) and TCR-αβ expression on nontransduced P3 cells (NT), P3 cells transduced with the TCR-α chain from T-cell clone N10 (+TCR-α N10), P3 cells transduced with the TCR-β chain from T-cell clone N10 (+TCR-β N10), and P3 cells with both the TCR-α and TCR-β chain from T-cell clone N10 (+TCR-αβ N10). (Bottom panel) NGFR- (TCR-β from N10) and TCR-αβ expression, also on nontransduced P3 cells (NT), P3 cells transduced with the TCR-α chain from T-cell clone N10 (+TCR-α N10), P3 cells transduced with the TCR-β chain from T-cell clone N10 (+TCR-β N10), and P3 cells with both the TCR-α and TCR-β chain from T-cell clone N10 (+TCR-αβ N10). Percentages of double-positive cells are indicated. FACS analysis after retroviral transduction of cells from RCD cell line P2 with the TCR-α and/or TCR-βchain from T-cell clone N10 showed similar results.