Retroviral introduction of exogenous TCR-β chains in RCD cell line P1 restores surface TCR-αβ expression. FACS analysis after retroviral transduction of cells from RCD cell line P1 with the TCR-α or TCR-β chain from T-cell clone N10. (Top panel) GFP- (TCR-α from N10) and TCR-αβ expression on nontransduced P1 cells (NT), P1 cells transduced with the TCR-α chain from T-cell clone N10 (+TCR-α N10), and on P1 cells transduced with the TCR-β chain from T-cell clone N10 (+TCR-β N10). (Bottom panel) NGFR- (TCR-β from N10) and TCR-αβ expression, also on nontransduced P1 cells (NT), P1 cells transduced with the TCR-α chain from T-cell clone N10 (+TCR-α N10), and on P1 cells transduced with the TCR-β chain from T-cell clone N10 (+TCR-β N10).