The TLR2/MyD88-dependent pathway is required for innate immune control of VV infection in vivo. (A) Production of IL-6. Wild-type C57BL/6 (WT), TLR2^−/−, or MyD88^−/− mice were administered intravenously with 1 × 10⁷ pfu VV or 30 μg LPS, and serum samples were harvested 6 hours later for secretion of IL-6 by ELISA. Serum from uninfected C57BL/6 mice was used as control (control). (B) Secretion of IFN-β. WT, TLR2^−/−, or MyD88^−/− mice were injected intravenously with VV and serum samples were assayed for secretion of IFN-β. Uninfected C57BL/6 mice were used as control (control). (C) Viral titer. 1 × 10⁷ pfu VV was injected intraperitoneally into female WT, TLR2^−/−, or MyD88^−/− mice and ovaries were harvested for measurement of viral load by plaque-forming assay 3 days later. Data represent viral titer as plaque-forming unit (pfu) per ovary. Error bars indicate SD.