Inhibition of SHP-2 catalytic activity does not destabilize p210 but attenuates cell survival and enhances serum starvation-induced apoptosis. Ba/F3 cells overexpressing catalytically inactive SHP-2 C459S and the vector control cells were transduced with Bcr-Abl as described in “Materials and methods.” (A) Whole-cell lysates prepared from the cell lines were examined for p210 and SHP-2 with IB using anti–c-Abl or anti–SHP-2 Abs. Exponentially growing cells were seeded into serum-free RPMI-1640 medium. Cell-survival rates at the indicated time points were determined by the MTS assay as described in Figure 1A. (B) Exponentially growing cells were starved of serum for 24 and 48 hours. Cells were harvested and processed for cell-cycle analyses. Percentages of apoptotic cells with sub-G1 DNA content were determined. Results shown are representative of 3 experiments.
