American Society of Hematology

Figure 6

$Treatment of primary MM cells with 17-DMAG induces apoptosis also in the presence of cells from the BMM. Primary MM cells were freshly isolated from 24 patients. (A-D) Primary MM cells were kept in medium with 10 ng/mL IL-6 (n = 20) or cultured either in the presence of BMSCs (n = 20) or OCs (n = 9) or HUVECs (n = 9). The primary MM cells were exposed for up to 7 days to 1 mM 17-DMAG. (E) 17-DMAG titration experiments with 3 BM-derived primary MM samples and with cells from a patient with PC leukemia (PCL). Primary MM cells were cocultured with BMSCs and exposed to different concentrations of 17-DMAG for 1 week. Viable cell fractions were assessed by annexin V–FITC/PI staining. Horizontal lines (A-D) indicate the median of each treatment cohort.$

Treatment of primary MM cells with 17-DMAG induces apoptosis also in the presence of cells from the BMM. Primary MM cells were freshly isolated from 24 patients. (A-D) Primary MM cells were kept in medium with 10 ng/mL IL-6 (n = 20) or cultured either in the presence of BMSCs (n = 20) or OCs (n = 9) or HUVECs (n = 9). The primary MM cells were exposed for up to 7 days to 1 mM 17-DMAG. (E) 17-DMAG titration experiments with 3 BM-derived primary MM samples and with cells from a patient with PC leukemia (PCL). Primary MM cells were cocultured with BMSCs and exposed to different concentrations of 17-DMAG for 1 week. Viable cell fractions were assessed by annexin V–FITC/PI staining. Horizontal lines (A-D) indicate the median of each treatment cohort.

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