Disruption of membrane rafts inhibits ALP-induced Fas/CD95 clustering and apoptosis. MM144 cells were untreated (control) or pretreated with MCD or filipin and then incubated with 10 μM edelfosine or perifosine for 12 hours and analyzed for Fas clustering by confocal microscopy (A), or for 24 hours, and examined for the percentage of apoptotic cells by flow cytometry (B). Images shown in panel A are representative of 3 independent experiments. Scale bar, 10 μm. Data shown in panel B are means ± SD of 3 independent determinations.