Oncogenic properties of cells with EEN overexpression. (A) Western blot analysis showing overexpression of EEN in stably transfected NIH3T3 and HL60 cells (2.3-fold in NIH3T3-EEN and 4-fold in HL60-EEN compared with the endogenous level). (B) Tumor formation in nude mice injected with NIH3T3-EEN. (C) Cell proliferation and cell-cycle distribution of HL60-GFP and HL60-EEN cells. The result of 1 representative experiment is shown. (D) Activation of AP-1 (left panel), but not CRE (right panel), by EEN. (E) Colonies generated per 10⁴ transduced BM cells were determined in first-, second-, and third-round cultures for MigR1-EEN construct, MigR1 empty vector, and nontransfected BM cells. GFP⁺ colonies were counted at the end of each round. Bars represent the mean plus or minus SD for 3 experiments.