Transcriptional regulation of the EEN gene by Sp1. (A) Deletion analysis of the activity of the EEN promoter in the Cos7 cell line. A significant difference is observed between EENP1 and other EEN promoter constructs. (B) Deletion analysis of the activity of the EEN promoter in the HL60 cell line shows similar results as in Cos7 cells. (C) EMSA showing the binding of Sp1 to the GC-stretch of EEN promoter with a DIG-labeled probe spanning the −107/−60 site. (D) Transcriptional activation of the EEN promoter by Sp1 in a dose-dependent manner. Each column represents the mean ± SD in 3 independent experiments.