Representative analysis of D816V KIT mutation in purified BM MCs and blast cells from 2 patients with SM-AHNMD. (A-B) Immunophenotypic characteristics of blast cells (red dots) and mast cells (black dots) in BM samples from patients 1 and 3 listed in Table 3, prior to FACS sorting. (C-F) Melting curves of wild-type (approximately 59°C; black line) and mutated (approximately 63°C; red line) KIT sequences from mast cells (C-D) and blast cells (E-F) from patients 1 (C-E) and 3 (D-F).