Melting curves and sequences of PCR products from patients with systemic mastocytosis carrying different KIT mutations. (A) D816V KIT mutation (melting curve at 63°C) found in a patient with ISM. (B) D816Y KIT mutation (56°C) found in a patient with MCL. (C) VI815-816 insertion (56°C) found in a patient with SM-ana. (D) I817V KIT mutation (54°C) found in a patient with WDSM. Comparison with typical D816V KIT mutation and wild-type sequence (melting curve at 59°C) are shown in all panels.