CD40L-stimulated EDI MoDCs fail to support the proliferation of allogeneic T cells. (A) Positively selected CD3⁺ cells (purity > 99%) from a healthy donor were mixed with increasing number of unrelated MoDCs from a healthy control (left panel) or patient with EDI (right panel) for 6 days in the presence of medium alone, CD40L (2.5 μg/mL), CD40L plus IFN-γ (2000 U/mL), or LPS (2.5 μg/mL). (B) CD3⁺ cell proliferation was evaluated after incorporation of triated thymidine by using a β scintillation counter. Data are shown as means ± SEM of counts per minute (cpm). (C) Addition of recombinant human IL-12 (10 ng/mL) rescues CD3⁺ lymphoproliferation in the presence of CD40L-stimulated EDI MoDCs. Data are shown as means ± SEM of one representative DC/T-cell ratio (1:20). *P < .05.