Histologic assessment of endothelial-cell chimerism. Representative histologic sections from the ischemic muscle at 14 days after the induction of hindlimb ischemia were incubated with VWF or isotype control antibodies. Shown is GFP (green) and VWF (red) expression in Tie2GFP mice (n = 3), nontransgenic FVB mice (nontransgenic control) (n = 3), or FVB mice following adoptive transfer of Tie2GFP blood mononuclear cells (adoptive transfer) (n = 3). Original magnification (× 400).