Figure 2.
Figure 2. Titration of BLyS-mediated enhancement of monocyte survival and effects of heat inactivation on biologic activity. Control (con; non-heat inactivated) and heat-inactivated (HI) (A) LBLyS (Calbiochem), (B) SBLyS (RDI), and (C) LPS were titrated over the indicated range of concentrations, and monocyte survival was determined on day 3. Data are representative of 3 independent experiments. (D) Apoptosis analysis of cells stimulated with control (con) or heat-inactivated 100 ng/mL LBLyS (HI) or 20 ng/mL LPS (HI) (data shown represent mean ± SD of 5 independent experiments); and (E) cells stimulated with control (con) or heat-inactivated 500 ng/mL SBLyS (HI) or 20 ng/mL LPS (HI) (data shown represent mean ± SD of 3 independent experiments). *When compared with Nil, P < .001. **When control LBLyS or SBLyS compared with heat-inactivated LBLyS or SBLyS, P < .001.

Titration of BLyS-mediated enhancement of monocyte survival and effects of heat inactivation on biologic activity. Control (con; non-heat inactivated) and heat-inactivated (HI) (A) LBLyS (Calbiochem), (B) SBLyS (RDI), and (C) LPS were titrated over the indicated range of concentrations, and monocyte survival was determined on day 3. Data are representative of 3 independent experiments. (D) Apoptosis analysis of cells stimulated with control (con) or heat-inactivated 100 ng/mL LBLyS (HI) or 20 ng/mL LPS (HI) (data shown represent mean ± SD of 5 independent experiments); and (E) cells stimulated with control (con) or heat-inactivated 500 ng/mL SBLyS (HI) or 20 ng/mL LPS (HI) (data shown represent mean ± SD of 3 independent experiments). *When compared with Nil, P < .001. **When control LBLyS or SBLyS compared with heat-inactivated LBLyS or SBLyS, P < .001.

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