Neutrophils from patients with VHL disease show modified rates of constitutive apoptosis. Peripheral-blood neutrophils were obtained from patients with genotyped VHL (▪) or matched healthy controls (▦) and cultured in either normoxia (19 kPa; A), normoxia, hypoxia (3 kPa), or anoxia (0 kPa; B) or in normoxia in the presence of the hydroxylase inhibitor DMOG (1 μM to 1 mM; C). Cells were assessed for apoptosis by morphologic appearance at 20 hours unless otherwise stated. Data in panel A represent the mean ± SEM of triplicate determinations for 4 control and 12 VHL patients. Data in panels B and C represent the mean ± SEM for 4 separate experiments each performed in triplicate (* P < .001).