Roles of P2Y₁₂, PI3Kβ, and PI3Kγ in stabilization of human thrombi. Human blood was flowed over VWF/collagen at 1000 s^–1 for 4 minutes. Disaggregation events were measured from thrombi during 10 minutes of secondary perfusion with plasma containing indicated inhibitors. (A) Effect of PI3K inhibitors TGX221 (1 μM) and AS252424 (1 μM). (B) Effect of P2Y₁₂ inhibitor AR-C69931MX (30 μM) alone or combined with TGX221 (1 μM), AS252424 (1 μM), or MRS2179 (100 μM). Data are means ± SEM (n = 4 experiments; *P < .05 vs vehicle).