Figure 4.
Figure 4. Continuous P2Y12 signaling is required for persistent αIIbβ3 activation. (A) Platelets in PPACK-anticoagulated PRP were activated with ADP (no stirring), and 2 minutes later inhibitors or antagonists were added (Figure 3). At indicated time points, activated αIIbβ3 was detected with FITC-PAC1, and integrin function was analyzed with OG-labeled fibrinogen. Histograms are given of FITC-PAC1 and OG-fibrinogen fluorescence distribution before and after AR-C69931MX addition. (B) Mean FITC-PAC1 fluorescence intensities ± SEM after incubation with inhibitor/antagonist (n = 3-4).

Continuous P2Y12 signaling is required for persistent αIIbβ3 activation. (A) Platelets in PPACK-anticoagulated PRP were activated with ADP (no stirring), and 2 minutes later inhibitors or antagonists were added (Figure 3). At indicated time points, activated αIIbβ3 was detected with FITC-PAC1, and integrin function was analyzed with OG-labeled fibrinogen. Histograms are given of FITC-PAC1 and OG-fibrinogen fluorescence distribution before and after AR-C69931MX addition. (B) Mean FITC-PAC1 fluorescence intensities ± SEM after incubation with inhibitor/antagonist (n = 3-4).

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