Hematopathologic, cytochemical, and histoimmunologic analysis of the N-RasD12–induced AML-like disease in mice. (A) Hematopathologic analysis of affected tissues in a representative NRASD12 mouse that succumbed to AML-like disease. Peripheral blood smears, bone marrow smears, and spleen touch preparations (from left to right) from vector control (top row) and a NRASD12 mouse (bottom row) were stained with the HEMA3 stains. Original magnification was × 630. Paraffin sections of liver and lung (rightmost columns) from vector control and NRASD12 mice were stained with hematoxylin and eosin. Images are magnified × 200. (B) Esterase staining for specific and nonspecific esterases (the left 2 columns) on WBCs from a diseased NRASD12 (top row) and a BCR-ABL (bottom row) mouse. The right 2 columns show a histoimmunochemical analysis of spleen sections from a diseased NRASD12 mouse with AML-like disease and a BCR-ABL mouse with CML-like disease, with anti-GFP (staining for leukemic cells) and anti-MPO antibodies, as indicated.