Characterization of the NRASD12-induced CMML-like disease in mice. (A) Morphology of the peripheral WBCs of a representative vector control, NRASD12 and BCR-ABL mouse. Images were taken under a Zeiss Axioskop microscope (Carl Zeiss, Thornwood, NY) using a Zeiss Microscope Camera System and a 40×/1.0 oil-immersion objective lens. Images were processed with Adobe Photoshop 7.0 software (Adobe, San Jose, CA). (B) Flow cytometry analysis on the peripheral WBCs freshly isolated from a representative diseased NRASD12 mouse, BCR/ABL mouse, and a vector control mouse as indicated. The level of GFP expression is shown along the x-axis, and the y-axis shows the expression of cell-surface markers specified over each column.