Neutrophil circulating and tissue half-life. (A) Neutrophil circulating half-life. This graph shows the mean percentage of E-GFP (percentage of total cells) neutrophils that remain in the circulation following their intravenous transfusion into SV129/black untreated, recipient mice. Flow cytometry was used to determine the percentage of E-GFP neutrophils present in the blood. From these findings we were able to calculate that the average half-life of a neutrophil is 8 hours long. Neutrophil t1/2 was calculated as previously described.16,17 Data are shown as mean ± SD (6-12 mice for each time point following transfusion). (B) Neutrophil tissue half-life. This graph shows the mean percentage of E-GFP (percentage of total cells) neutrophils that occupy the spleen and lung tissue compartments of E-GFP mice on days after irradiation and no BMT. Flow cytometry was used to determine the percentage of E-GFP neutrophils present in single-cell suspensions of the lungs, spleen, and oral cavity. Neutrophil tissue half-life was calculated as previously described and found to be, on average, 2 days and 6 days long for the spleen and lungs, respectively.16,17 Data are shown as mean ± SD (3-15 mice for each day after irradiation).
