Examples of the application of TCR multiplex PCR for detection of immunodominant clonotypes in BM biopsies. Multiplex VB PCR combined with clonotype sequencing allowed for the detection of immunodominant clonotypic expansions in archived bone marrow biopsies. VB indicates T-cell receptor VB chain; CDR3, complementarity determining region 3; JB, joining region of the TCR B chain; and CB, constant B chain. All immunodominant BM-derived clonotypes are shown in Table 7. Images were obtained via digital microscopy using an Olympus BX41 microscope (Olympus America, Melville, NY) equipped with a 40×/0.75 NA UPlan objective lens. Slides were stained with hematoxylin and eosin. Images were captured using a Digital Camera Model no. 11.2 Color Mosaic (Diagnostic Instruments, Sterling Heights, MI), and imaging software included Spot Basic 4.1.3 (Diagnostic Instruments).