Figure 2.
CCL21 induces microvillar collapse in wt but not in DOCK2–/–Tlymphocytes. (A) Micrographs from field emission scanning electron microscopy (SEM) of wt and DOCK2–/– T lymphocytes, intact or stimulated for 1 minute with CCL21 (300 ng/mL). Bar = 2 μm. Micrographs are representative of 40 wt cells and 38 DOCK2–/– cells analyzed. (B) Resistance to detachment developed by T cells arrested on either VCAM-1 (left) or ICAM-1 (right) coimmobilized with CCL21 as in Figure 1D-E subjected to continuous application of low shear (0.5 dyne/cm2) up to 1 minute and then subjected to abrupt detachment by 10-fold higher shear stress for 5 seconds. Mean values ± range determined in 2 representative fields are presented.
