Effects of S 36578-2 on HUVECs and CDC.HMEC-1 cells spread on fibronectin. S 36578-2 was added to cells 3 hours after seeding in experimental medium. (A) Phase contrast micrographs were taken 24 hours after treatment. Scale bar represents 100 μm. Results from a representative experiment, repeated at least 3 times, are shown. (B) Floating and attached cells were collected 24 hours after treatment. Apoptosis was assessed by flow cytometry after annexin V and propidium iodide staining. Results of 1 of 2 experiments with similar results are shown. (C) Procaspase-8, procaspase-9, cleaved caspase-3, and PARP were detected by Western blotting. Erk1/2 was used as loading control. *Nonspecific detection of BSA. Results from 1 of 5 representative experiments for HUVECs and 1 of 4 representative experiments for CDC.HMEC-1 cells are shown. (D) Cells were processed for F-actin detection 30 minutes after the addition of S 36578-2. Scale bar represents 20 μm.