ShRNATAL1 lentiviral vector efficiently decreases TAL1 expression in hematopoietic cells. (A) Lentiviral construct for human shRNATAL1 and GFP expression. Bold characters correspond to sense and antisense oligonucleotides used for shRNATAL1 design. (B) Transduction efficiency assessed by GFP expression in Jurkat cells and CD34⁺ cells after shRNActrl and shRNATAL1 vector transduction. (C) Western blot analysis of human TAL1 protein in total unsorted transduced cells. β-actin is used as protein loading control.