CD8 T cells from WT or IFN-γ–deficient mice are equally efficient in suppressing Th2 response and tissue eosinophilia. One day before grafting, CD8-deficient B6 mice were intravenously injected with CD8 T cells from either WT or IFN-γ–deficient B6 mice. Mice then received a skin graft from semi-allogeneic (CB6F1) mice. At day 10, grafts and draining lymph nodes were harvested. (A) Purified CD4⁺ T cells were cultured (2 × 10⁵ cells/well) in the presence of irradiated allogeneic BALB/c splenocytes (3 × 10⁵ cells/well). Proliferation (A) and cytokine production (B) were measured at 72 hours of culture. Results are expressed as mean plus or minus SEM of 5 to 7 mice per group. (C) The percentage of eosinophils among total cell infiltrates was evaluated as described in “Materials and methods” (mean ± SEM of 3-4 mice per group). Relative levels of IL-4 (D), IFN-γ (E), and TCRβ (F) mRNA were analyzed by real-time quantitative reverse transcriptase–polymerase chain reaction (RT-PCR) from skin graft cDNA and normalized to the HPRT mRNA. Data are pooled from 2 independent experiments with 9 to 12 mice per group. Statistical significance of difference between groups was evaluated by the Mann-Whitney U test (*P < .05; **P < .01; ***P < .005; NS, not significant; ND, not detectable).