American Society of Hematology

Figure 5.

$Figure 5. Flow cytometric analysis of siat1ΔP1 and WT bone marrow cells. Bone marrow cells were labeled with FITC-1A8 (anti-Ly6G), PE-7/4 (anti–polymorphonuclear cell, 40-kDa antigen), and biotinylated SNA, as described in “Flow cytometric profiling of inflammatory cells.” Unfractionated bone marrow cells (Total), and differentially flow-sorted populations R1, R2, and R3 were analyzed for SNA binding. SNA binding profiles of wild-type (dashed lines) and Siat1ΔP1 (solid lines) cells are compared.$

Flow cytometric analysis ofsiat1ΔP1 and WT bone marrow cells. Bone marrow cells were labeled with FITC-1A8 (anti-Ly6G), PE-7/4 (anti–polymorphonuclear cell, 40-kDa antigen), and biotinylated SNA, as described in “Flow cytometric profiling of inflammatory cells.” Unfractionated bone marrow cells (Total), and differentially flow-sorted populations R1, R2, and R3 were analyzed for SNA binding. SNA binding profiles of wild-type (dashed lines) and Siat1ΔP1 (solid lines) cells are compared.

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