Figure 2.
Figure 2. RNAi by transfection with shRNA expression vectors. (A) Schematic of the expression vectors (pENTR/shRNA) encoding shRNAs of Nef366 or lacZ, designated as pENTR/shNef366 and pENTR/shLacZ, respectively, in which expression is driven by the human U6 polymerase III promoter. (B) Nef-expressing HeLa-CD4 cells were transfected either with 1.0 μg pENTR/shNef366 or pENTR/shLacZ and cells were harvested 72 hours after transfection. Total RNA was extracted and analyzed by qRT-PCR. The data represent the average ± SD of 3 independent experiments.

RNAi by transfection with shRNA expression vectors. (A) Schematic of the expression vectors (pENTR/shRNA) encoding shRNAs of Nef366 or lacZ, designated as pENTR/shNef366 and pENTR/shLacZ, respectively, in which expression is driven by the human U6 polymerase III promoter. (B) Nef-expressing HeLa-CD4 cells were transfected either with 1.0 μg pENTR/shNef366 or pENTR/shLacZ and cells were harvested 72 hours after transfection. Total RNA was extracted and analyzed by qRT-PCR. The data represent the average ± SD of 3 independent experiments.

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