Live WASp-Ile294Thr macrophages exhibited abnormal shape and motility. (A-C) Comparison of area, elongation factor, and dispersion factor for normal, WASp-deficient (–), and WASp-Ile294Thr macrophages. (D,E,F) Representative composite images of normal, WASp-deficient, and WASp-Ile294Thr macrophages, respectively. Dashed lines represent the outline of the cell body. (G) Quantification of podosome turnover. *P < .05; **P < .01; ***P < .001. Error bars indicate 1 SE.