Representative sections of bone marrow from children with severe malarial anemia. (A-C) Highly abnormal erythropoiesis (irregular, bi-, and multinucleated erythroid cells) in association with Hz (arrows). (D) Hz predominantly inside myeloid cells (hematoxylin and eosin staining; original magnification, × 1000). Cells were viewed with a Nikon Eclipse E600 microscope equipped with a Nikon 100 ×/1.40 numeric aperture oil-immersion objective lens (Nikon, Surrey, United Kingdom) and a Zeiss Axiocam (Zeiss, Göttingen, Germany). Axiovision 3 software (Zeiss) was used to acquire images, and ImageJ software (http://rsb.info.nih.gov/ij) was used to process them.